9^th World Biomarkers Congress

Antiae is a recently discovered novel external organelle responsible for the gliding motility in bacteria. Th e antiae composed of plural thin fibers entangled in plexa that may be 10-20 folds of the cellular size, and spin in a coherent, unison rotation around the cell cylinder causing a high-sequence trembling that results a smooth slow gliding.  DAPI stain of strain A7P-90mT demonstrated the absorbance of the stain by the antiae that indicates on possible RNA or DNA molecules involvement in cellular constitution. UV absorbency pick of the living culture was at 300 nm as well as a filtrated through 0.2 μm fi lter liquid containing antiae. Here we discuss the results of the proton, sodium pumps and ATPase inhibition effects on the cellular motion. We also present the results of RNase and DNase treatment along with the thiamine bromide stain with UV imaging.  We also discuss the chemical composition of the antiae with increased amounts of fluorine and silicon according to X-ray spectrophotometry. Presence of the antiae was shown in other bacterial species and Archaea, as well as in viruses. Histopathology of sarcoma and other malignant cancers demonstrated presence of antiae in cell-free areas aff ected by cancer tissues that makes them an important biomarker in cytodiagnostics.

Keywords: bacterial gliding, external organelle antiae, cytodiagnostics, pathohistology, X-ray spectroscopy.