Amr Al-Haidari
Lund University, Sweden
Title: MiR-155-5p positively regulates colon cancer cell migration by targeting RhoA
Biography
Biography: Amr Al-Haidari
Abstract
Colorectal cancer is one of the most commonly diagnosed cancers worldwide and most of death-related cases are due to metastasis. It is becoming interestingly obvious that mciroRNAs play a pivotal role in the tumorgenesis of different tumors including colon cancer. MiR-155 has been shown to regulate key proteins involved in the metastasis process. In our present study, we shed the light on the regulation mechanism of chemokine-induced colon cancer cell migration by miR-155 in serum starved HT-29 colon cancer cells. MiR-155-5p knockdown experiments using antagomiR-155-5p decreased HT-29 CCL17-induced colon cancer cells migration. Using GLISA assays, knocking down miR-155-5p demonstrated significant inhibition of CCL17-induced activation of RhoA suggesting that RhoA could be a potential target for miR-155-5p in HT-29 colon cancer cells. Bioinformatics analysis predicted a putative binding AU-rich elements regulatory site in 3´-UTR of RhoA. MiR-155-5p:RhoA binding was verified using target site blockers and functionally validated using RIP assays indicating that RhoA-positive regulation is mediated by the AU-rich elements present 3´-UTR of RhoA mRNA. These results showed that miR-155-5p positively regulates RhoA in starved HT-29 colon cancer cells and inhibiting miR-155-5p could be a useful strategy to antagonize colon cancer metastasis.
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