Husham Y M Ali Bayazed
University of Zakho, Iraq
Title: Concentration levels of IL -10 and TNF-α cytokines in patients with HPV DNA positive and negative cervical lesions
Biography
Biography: Husham Y M Ali Bayazed
Abstract
Objective: The present study was performed to assess the immune response in women with HPV DNA positive and negative cervical lesions.
Methods & Patients: Eighty women with cervical lesions (age range of 25-70 years) were studied. The lesions were cytologically classified into 4 groups: ASC-US (20), CINI (30), CINII-III (16), and cervical carcinoma (14), prior to HPV DNA detection. Estimation of IL-10 and TNF-α cytokines was performed via enzyme linked immunosorrbent assay (ELISA) technique in cervical secretions and serum and PCR screening kits were utilized to detect HPV DNA on cervical smears.
Results: The detected levels of IL-10 (mean±SE) concentration in cervical secretions of patients with HPV DNA positive and negative states and control group were 88.73±16.90 pg/ml, 24.00±2.84 pg/ml and 8.27±0.59 pg/ml respectively with significant differences (p<0.05), while levels of TNF-α in cervical secretion of the studied groups were 12.18±3.49 pg/ml, 9.90±0.73 pg/ml and 7.90±0.87 pg/ml respectively with non significant differences. The detected levels of IL-10 in cervical secretions of HPV DNA positive cases (88.73±16.90 pg/ml) were significantly higher than in the sera (13.69±2.41 pg/ml) (p<0.05), while the levels of TNF-α in their cervical secretions (12.18±3.49 pg/ml) was slightly raised than in their sera (11.5 9±3.14 pg/ml) with non significance differences.
Conclusions: A raised levels of both IL-10 and TNF-α in secretions of HPV DNA positive women with different cervical lesions were detected. However, the observed higher levels of IL-10 than TNF-α indicate down-modulation of tumor-specific immune response to HPV infected lesions via significant raised concentrations of the first cytokine than the second one. Therefore, this phenomenon seems to provide a tumor progressive microenvironment by the immunosuppressant properties of IL-10 with minimal antitumor activity of TNF-α.